Iago's Motives In Othello

Ruth Frenkel MIC 220 Challenge questions set #2 Due April 3, 2002 SID# 601-50-5380 1.2.          thither be a multitude of reasons that the Escherichia coli did non produce the interferon after beingness alter. First, the E. coli cells might not move over accepted the plasmid DNA during the displacement process. If the electroporation was use, the potentiality might have been too high-pitched and irreversibly damaged the cell membrane, thereby rendering the cells incompetent. Conversely, the voltage used might have been too low and not have induced micropores in the cell membrane large ample for the uptake of foreign DNA. For E. coli the voltage hustle for flourishing displacement by electroporation is 250 to 4,000 V/cm. Voltages above or on a lower floor this range ordaining not result in transformed cells, and so will not evoke interferon. Another interpretation for the neediness of interferon production is that the plasmid did not contain a tan gible promoter upstream of the inserted interferon component. Without a immobile promoter, the E. colis RNA polymerase will not bind to the recombinant plasmid and thus will not transcribe the interferon cistron. On the other hand, if there was a sanitary promoter, the protein could have been over-transcribed and the host cells machinery would be overloaded, resulting in inadequate modality of the interferon. Yet another reason for the absence of interferon expression is that the inserted gene fragment did not have a leader sequence, likewise known as a type peptide. These sequences are typically located at the N-terminus of a protein and facilitate the transport of express proteins into the periplasm. This exportation renders the expressed foreign protein more stable than if it lie down in the E. colis cytol. If the interferon gene was not attached to a call for peptide, then it may have been degraded in the cytoplasm by endogenous exonucleases. Also, since interferon is... ! If you want to get a proficient essay, order it on our website: OrderEssay.net

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