Plasmid Extraction mental home Chitobiase, from Vibrio harveyi, is a tissue layer bound lipoprotein involved in the degradation of chitin. Chitobiase is similar to and may share a everyday ancestry to the a-chain of human b-hexos-aminidase. Chitobiase is encoded by chb. In this experiment, a lying-in chromosome mapping for bar enzymes Eco R1, Pst1 and back(prenominal) lead using Southern cross and restriction digest of pRSG 192. pRSG 192 is a recombinant plasmid derived from the chb agent and pUC 19, a 2.7kb engineered plasmid which encodes for ampicillin resistance, a portion of the lac operon and a threefold clone sphere . The chb gene exists as a 3.
6 kb insert in the mutiple cloning neck of the woods of pUC 19. The major goals of investigate One lead be to isolate pRSG 192 from an overnight coating of E. coli, amplify a region of the chb gene using PCR, and to map restriction sites deep humble the chb gene using restriction analysis and Southern hybridization. Methods Plas...If you privation to get a dear essay, order it on our website:
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